Photo of Amanda K. McCullough, Ph.D.

Amanda K. McCullough Ph.D.

  • (503) 494-9958
    • Associate Professor Oregon Institute of Occupational Health Sciences
    • Molecular and Medical Genetics Graduate Program School of Medicine
    • Cancer Biology Graduate Program School of Medicine

Dr. McCullough received her doctoral degree in cellular and molecular biology from the University of Vermont. She completed postdoctoral training at Oregon Health & Science University and the University of Texas Medical Branch in Galveston, Texas. She was an Assistant Professor in the Department of Human Biological Chemistry and Genetics at the University of Texas before joining Center for Research on Occupational and Environmental Toxicology (CROET) at OHSU in August 2003.Research in the McCullough laboratory is focused on the biochemical mechanisms of DNA repair systems and the regulation and roles of DNA repair in cellular responses to environmental stress. Ultimately, we are interested in correlating alterations in these systems with human cancers, aging and other disease states. Currently our research is focused on:  1) cellular pathways for the tolerance and repair of DNA-protein crosslinks; 2) biochemical mechanisms and therapeutic applications of ultraviolet (UV) light-induced DNA damage-specific glycosylases; and 3) mechanisms and regulation of human oxidative DNA damage-specific repair and how defects in these pathways contribute to colorectal cancers and metabolic disease.

Education

  • Ph.D., University of Vermont School of Medicine, Burlington Vermont 1992

Publications

  • "NEIL1 protects against aflatoxin-induced hepatocellular carcinoma in mice." Proceedings of the National Academy of Sciences of the United States of America  In: , Vol. 114, No. 16, 18.04.2017, p. 4207-4212.
  • "Enhanced sensitivity of Neil1 mice to chronic UVB exposure." DNA Repair  In: , Vol. 48, 01.12.2016, p. 43-50.
  • "DNA polymerase ζ limits chromosomal damage and promotes cell survival following aflatoxin exposure." Proceedings of the National Academy of Sciences of the United States of America  In: , Vol. 113, No. 48, 29.11.2016, p. 13774-13779.
  • "Catalysts of DNA Strand Cleavage at Apurinic/Apyrimidinic Sites." Scientific Reports  In: , Vol. 6, 28894, 01.07.2016.
  • "Small Molecule Inhibitors of 8-Oxoguanine DNA Glycosylase-1 (OGG1)." ACS Chemical Biology  In: , Vol. 10, No. 10, 16.10.2015, p. 2334-2343.
  • "BLM protein mitigates formaldehyde-induced genomic instability." DNA Repair In: , Vol. 28, 01.04.2015, p. 73-82.
  • "Inhibition of DNA glycosylases via small molecule purine analogs." PLoS One In: , Vol. 8, No. 12, e81667, 09.12.2013.
  • "Regulation of DNA glycosylases and their role in limiting disease." Free Radical Research In: , Vol. 46, No. 4, 04.2012, p. 460-478.
  • "Formaldehyde-induced genome instability is suppressed by an XPF-dependent pathway." DNA Repair In: , Vol. 11, No. 3, 01.03.2012, p. 236-246.
  • "Modulation of the processive abasic site lyase activity of a pyrimidine dimer glycosylase." DNA Repair  In: , Vol. 10, No. 10, 10.10.2011, p. 1014-1022.
  • "γ-Hydroxy-1, N 2-propano-2′-deoxyguanosine DNA adduct conjugates the N-terminal amine of the KWKK peptide via a carbinolamine linkage." Chemical Research in Toxicology In: , Vol. 24, No. 7, 18.07.2011, p. 1123-1133.
  • "Variable penetrance of metabolic phenotypes and development of high-fat diet-induced adiposity in NEIL1-deficient mice." American journal of physiology. Endocrinology and metabolism  In: , Vol. 300, No. 4, 04.2011.
  • "TAT-mediated delivery of a DNA repair enzyme to skin cells rapidly initiates repair of UV-induced DNA damage." Journal of Investigative Dermatology In: , Vol. 131, No. 3, 03.2011, p. 753-761.
  • "Minor groove orientation of the KWKK peptide tethered via the N-terminal amine to the acrolein-derived 1, N 2-γ- hydroxypropanodeoxyguanosine lesion with a trimethylene linkage." Biochemistry In: , Vol. 49, No. 29, 27.07.2010, p. 6155-6164.
  • "Modulation of UvrD helicase activity by covalent DNA-protein cross-links." Journal of Biological Chemistry In: , Vol. 285, No. 28, 09.07.2010, p. 21313-21322.
  • "Cellular pathways for DNA repair and damage tolerance of formaldehyde-induced DNA-protein crosslinks." DNA Repair In: , Vol. 8, No. 10, 02.10.2009, p. 1207-1214.
  • "The basal levels of 8-oxoG and other oxidative modifications in intact mitochondrial DNA are low even in repair-deficient (Ogg1/Csb) mice." Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis  In: , Vol. 625, No. 1-2, 01.12.2007, p. 155-163.
  • "Human polymorphic variants of the NEIL1 DNA glycosylase." Journal of Biological Chemistry  In: , Vol. 282, No. 21, 25.05.2007, p. 15790-15798.
  • "Uncoupling of nucleotide flipping and DNA bending by the T4 pyrimidine dimer DNA glycosylase." Biochemistry  In: , Vol. 45, No. 47, 28.11.2006, p. 14192-14200.
  • "Structure of T4 Pyrimidine Dimer Glycosylase in a Reduced Imine Covalent Complex with Abasic Site-containing DNA." Journal of Molecular Biology  In: , Vol. 362, No. 2, 15.09.2006, p. 241-258.
  • "The metabolic syndrome resulting from a knockout of the NEIL1 DNA glycosylase." Proceedings of the National Academy of Sciences of the United States of America  In: , Vol. 103, No. 6, 15.02.2006, p. 1864-1869.
  • "Reaction intermediates in the catalytic mechanism of Escherichia coli MutY DNA glycosylase." Journal of Biological Chemistry  In: , Vol. 279, No. 45, 05.11.2004, p. 46930-46939.
  • "Identification of critical residues required for the mutation avoidance function of human MutY (hMYH) and implications in colorectal cancer." Cancer Letters In: , Vol. 205, No. 1, 08.03.2004, p. 89-95.
  • "Chlorella virus pyrimidine dimer glycosylase excises ultraviolet radiation- and hydroxyl radical-induced products 4,6-diamino-5-formamidopyrimidine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine from DNA." Photochemistry and Photobiology  In: , Vol. 75, No. 2, 01.02.2002, p. 85-91.
  • "hMYH cell cycle-dependent expression, subcellular localization and association with replication foci : Evidence suggesting replication-coupled repair of adenine:8-oxoguanine mispairs." Nucleic Acids Research In: , Vol. 29, No. 13, 01.07.2001, p. 2802-2809.
  • "The reaction mechanism of DNA glycosylase/AP lyases at abasic sites." Biochemistry In: , Vol. 40, No. 2, 16.01.2001, p. 561-568.
  • "AP lyases and dRPases : Commonality of mechanism." Mutation Research - DNA Repair  In: , Vol. 459, No. 1, 16.02.2000, p. 43-53.
  • "Intron conservation in a UV-specific DNA repair gene encoded by chlorella viruses." Journal of Molecular Evolution In: , Vol. 50, No. 1, 2000, p. 82-92.
  • "Initiation of base excision repair : Glycosylase mechanisms and structures." Annual Review of Biochemistry  In: , Vol. 68, 1999, p. 255-285.
  • "Characterization of a novel cis-syn and trans-syn-II pyrimidine dimer glycosylase/AP lyase from a eukaryotic algal virus, Paramecium bursaria chlorella virus-1." Journal of Biological Chemistry  In: , Vol. 273, No. 21, 22.05.1998, p. 13136-13142.

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