Histopathology Shared Resource - LASER CAPTURE MICROSCOPY
The core facility houses a PIXCELL II laser capture microscope(Arcturus Engineering) that can be used to microdissect small clusters of cells, or even single cells, for use in DNA, RNA or protein analyses. Cells can be dissected from either cryostat or paraffin sections that have been stained with H&E, a special stain, or even an immunostain.
There is no charge for time on the scope. Users must provide their own supplies.
Laser Capture Microscopy Procedure:
The procedure is simple and rapid. Training time is less than one hour, and a trained user can dissect from one to several thousand cells within a matter of minutes. This procedure is outlined below.
The PIXCELL II scope has an integrated digital image capture unit, allowing one to record "before" and "after" images of the dissected cells. In the example shown below, prostate tumor glands were dissected away from adjacent normal glands