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Histopathology Shared Resource - IMMUNOHISTOCHEMISTRY

The Histopathology Shared Resource has an automated Dako immunostainer with a capacity of 48 slides per run, allowing high throughput staining. Hand staining is also employed. Services provided include:

  1. Testing and titering new antibodies, both monoclonal and polyclonal. The latter should be either a purified IgG fraction or affinity-purified. (Straight serum is not suitable for immunostaining).
  2. Optimization of staining to enhance detection and reduce background.
  3. Epitope retrieval techniques (pre-treatment with heat and/or proteases)
  4. Specialized secondary detection reagents
  5. Avidin/biotin blocking steps
  6. Double- and triple-antibody stains using multiple chromagens.

Partial list of antibodies previously used:

Smooth Muscle Actin Muscle Specific Actin BCL-2 CD 134
Cathepsin C Cox-2 KI-67 (MIB-1) CD 45R
CD 34 CD 45 RA CD 35 RO CD 54
C-kit (CD 117) Collagen III Desmin CD4
Factor VIII Estrogen Receptor p53 CD8a
Progesterone Receptor S 100 Vimentin CD8b
Cathepsin B Chymase GFP Smad4
CD 3 CD 4 CD11 b Ski
CD 19 CD 26 CD 31 SCF
CD 73 CD 81 c-erbB-2 TGF beta RII
hCG anti-Corticotropin Releasing Factor c-Myc Raf-B
anti-Cytokeratin Epidermal Growth Factor Receptor FGFR-3 anti-Rad51
FGF-2 FAS Ligand Fibronectin Rb
FLAG anti-Glucagon HER-2 TGF beta RI
Integrin IL-1 beta Ly-6G VEGF
LCA MK MMP-9 NKR-P1A
SMMS-1 anti-Myosin NF2 p27
p16 Phospho-p44/42 p44-42 MAP Kinase ED1
Phospho-Akt Phospho-EGF Receptor Phospho-mTOR Thrombospondin-1
Phospho-p70 Phospho-PKC Phospho-Rb SnoN
Phospho-Src PDGF-R beta p21 anti-APAF-1/NB
PCNA p16 MiTF MOMA-1
CD 11 b/c CD 25 (IL-2R alpha chain) RT1B  

For charges related to immunhistochemistry, please refer to Histology Services.

To request immunohistochemistry services, please contact:

Christopher Corless, MD, Ph.D

Contacts:

Christopher Corless, M.D., Ph.D
ph. (503)402-2827;
OHSU pager 12375

Cara Poage
ph.(503)220-8262
VA ext. 5-4127

Carolyn Gendron
ph.(503)220-8262
VA ext. 5-4127

For detailed location information: Contact Us