KIT Mutations In Mastocytosis
Test Description
Screening for KIT kinase gene mutations in cases of mast cell disease is performed using an allele-specific real-time PCR assay that is specific for the D816V substitution. The sensitivity of this assay is estimated at 2% mutant cells.
Specimens Suitable For Testing
| Diagnosis | Bone marrow core* | Bone marrow clot | Bone marrow aspirate# | Blood^ | Skin bx | GI bx |
| Mastocytosis | √ | √ | √ | No | √ | √ |
Specimen Processing Guidelines:
*Bone marrow cores: formalin-fixed is OK. Do not use fixatives such as Zenker’s, B5, Hollande’s.
#Aspirates: Aspirates should be shipped within 24 hrs. Please avoid shipping on Friday. EDTA or citrate tubes only. No heparin tubes.
How To Order This Test
Send the following items to Dr. Corless at the address below.
- Completed Test Requisition Form (including patient’s billing information).
- A paraffin block or 15 unstained slides (uncharged slides preferred) representative of the tumor.
- A copy of the original pathology report.
Shipping address:
Knight Diagnostic Laboratory
2525 SW 3rd Ave.
Ste. 350
Portland, OR 97201
Ph: 503-494-5400
CPT Codes
| CPT Code | |
| Lysis of Cells, Paraffin* | 83907 |
| DNA extraction* | 83890 |
| Enzymatic digestion* | 83892 |
| Allele Specific PCR | 83905 |
| Professional Fee** | 83912 |
*Lysis of cells not performed for bone marrow aspirate specimens. Lysis extraction and digestion are not performed for purified DNA specimens.
**Note: The professional fee is only for molecular testing. There is no pathology consultation fee.
For additional information, contact Knight Diagnostic Laboratory Services Center at 503-494-5400.
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