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GI Stromal Tumor (GIST)

Test Description

KIT and PDGFRA gene exons are amplified by PCR and screened for mutations using denaturing HPLC (Transgenomic WAVE™ system), or high resolution melting curve analysis. Detected mutations are confirmed by standard bi-directional DNA sequencing. Testing is performed using a serial approach depending on primary tumor location.

Initial testing
    Gastric tumor:    KIT Exon 11
    All other sites:    KIT Exons 9 & 11

Additional testing performed if initial testing is negative
    KIT exons (9), 13 & 17
    PDGFRA exons 12, 14 & 18

How To Order This Test

To order KIT and PDGFRA mutation screening, send the following items to Dr. Corless at the address below.

  1. Completed Test Requisition Form (including patient’s billing information).
  2. A paraffin block or 15 unstained slides (uncharged slides preferred) representative of the tumor.
  3. A copy of the original pathology report.

Shipping address:

Knight Diagnostic Laboratory
2525 SW 3rd Ave.
Ste. 350
Portland, OR 97201
Ph: 503-494-5400

Shipping Information: Blocks and slides may be shipped at room temperature.

CPT Codes

  CPT Code
Lysis of Cells, Paraffin 83907
DNA extraction 83890
Enzymatic digestion 83892
PCR (per exon) 83898
DNA sequencing 83904
*Professional Fee 83912

*Note: The professional fee is only for molecular testing. There is no pathology consultation fee.

For additional information, contact Knight Diagnostic Laboratory Services Center at 503-494-5400.

Links: Back to Knight Diagnostic PTRL, Research Tests Available, GIST Test Descriptions, GIST Background Information