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Panel 1: Histochemical Stains on Fresh Frozen Skeletal Muscle Specimens
  • Frozen Skeletal Muscle Specimens
  • Gomori Modified Trichrome
  • Succinyl Dehydrogenase
  • NADH
  • Cytochrome Oxidase
  • PAS with Diastase
  • Oil-Red-O
  • Acid Phosphatase
  • Alkaline Phosphatase
  • Non-Specific Esterase
  • ATPase (pH 9.4 and 4.5)
Panel 2: Immunohistochemical Stains for Dystrophies
  • Spectrin
  • Dystrophin (N-terminal, C-terminal, Rod-domain)
  • Alpha, beta, delta, and gamma- Sarcoglycans
  • Beta-Dystroglycan
  • Utrophin
  • Merosin
  • Merosin-M
  • Emerin
  • Caveolin-3
  • Dysferlin
Panel 3: Inflammatory Myopathy Panel
  • CD3
  • CD20
  • CD8
  • CD4
  • CD68
  • Ubiquitin
  • MHC-Class 1
  • Congo Red with polarization and
       fluorescence microscopy
Panel 4: Metabolic Myopathy Panel
  • Modified SDH/COX double stain
  • Myophosphorylase
  • Myoadenylate Deaminase
  • PAS on plastic-embedded muscle sample
  • Electron microscopy for abnormal mitochondria,
       excess glycogen and lipid

Click here for a complete immunohistochemical antibody list.

Peripheral nerve:

All peripheral nerve samples are fixed in glutaraldehyde for electron microscopy, as well as in paraffin for light microscopy. For suspected cases of monoclonal gammopathy, fresh tissue is saved for immunofluorescence studies.

Skin biopsy for Intraepidermal Nerve Fiber Density evaluation with PGP 9.5:

For suspected cases of small-fiber neuropathy, PGP 9.5 immunostain is employed on skin biopsies for intraepidermal nerve fiber density evaluation.

Laboratory of Neuromuscular Pathology

We process and perform full diagnostic analysis of skeletal muscle and peripheral nerve specimens from adult and pediatric patients at this facility. We routinely accept and process specimens from the OHSU hospitals, other institutions and clinics in Oregon and from other states as express-mailed fresh tissue samples, and/or fixed samples for paraffin embedding and electron microscopy. Common diagnostic inquiries include inflammatory myopathies (polymyositis, dermatomyositis, inclusion body myositis), various dystrophies, metabolic myopathies (mitochondrial myopathy, acid maltase, myophosphorylase deficiency, and others), drug-induced myopathy (statin myopathy and others) and motor neuron diseases. For a complete and accurate evaluation of neuromuscular diseases, appropriately prepared and shipped fresh samples are critical. For handling fresh muscle and nerve biopsy specimens and shipping instructions please refer to our Protocols for Muscle and Nerve Biopsies. We have a comprehensive histochemistry panel for evaluation of neuromuscular diseases (1), and immunohistochemical panels for dystrophies (2) and inflammatory myopathies (3). In selected cases, we also perform ultrastructural analysis of muscle and peripheral nerve specimens at our electron microscope facility.