Molecular Pathology Supervisors: Richard Press. MD, PhD Location: This rotation occurs at the OHSU DNA Diagnostic Lab, Marqam Plaza Building Duration: 1 month Goals & Objectives:     Pathology resident education in molecular pathology stems from their required 1month rotation through the DNA Diagnostic Lab, a joint venture of the Pathology and Genetics departments. During this rotation the residents are exposed to the unique language, technology, and predictive interpretive aspects of molecular pathology through a combination of didactic lectures, teaching conferences, independent study, clinical-pathologic presentations, and hands-on lab experience. The interpretive reports and consultative activities that the resident will participate in include the clinical interpretation of any of the DNA lab tests listed below. DNA-based lab tests are not a significant part of any other Pathology resident rotation. DNA Diagnostic Lab Test        Diagnostic Methods                             Annual # of Tests Bcr/Abl                                    Quantitative real-time RT-PCR.                    543 CMV                                       Quantitative real-time PCR                         1196 Muscular Dystrophy                  PCR/Southern Blot                                        25 Bone Marrow Engraftment        Southern Blot (VNTR)                                 139 Factor V Leiden                        Real-time PCR / FRET                                852 Fragile X                                   Southern Blot/PCR                                      322 Friedreich ataxia                        PCR                                                              16 Hemochromatosis                      Real-time PCR / FRET                                627 HCV Qualitative                        RT-PCR-ELISA                                         317 HCV Genotype                         RT/PCR/LIPA                                            251 HCV Quantitative                      RT-PCR-ELISA                                         474 HIV viral load                            RT-PCR-ELISA                                         993 HIV ultra viral load                    RT-PCR-ELISA                                          237 Huntington Disease                    PCR/PAGE/blotting                                       45 Mitochondrial deletion                Southern Blot                                                  7 MELAS                                    PCR/PAGE                                                   18 MERRF                                    PCR/PAGE                                                   12 NARP                                       PCR/PAGE                                                   10 MTHFR C677T                        PCR/RFLP                                                    45 Myotonic Dystrophy                  Southern Blot/PCR                                        34 Parentage                                  Southern Blot (VNTR)                                 220 Prader-Willi Angelman               Southern Blot                                                25 Prothrombin G20210A              Real-time PCR / FRET                                634 SCA1                                        PCR/PAGE/blotting                                        7 SCA3                                        PCR/PAGE/blotting                                        7 SMA                                         PCR/RFLP                                                   24 Graduated Responsibility, Supervision and Evaluation: 1.    Consultation with clinical staff on proper utilization and interpretation of molecular diagnostic         tests. 2.    Consultation with laboratory staff on the clinical/pathological correlates of the various disease        entities being evaluated. 3.    Acquiring and disseminating clinical and familial information on the patients being evaluated in the        lab. 4.    Regular reviews of the primary laboratory data for interpretation, oral presentation to the        laboratory director, and write-up of an interpretive report. 5.    The privilege of consulting with the clinical staff on proper utilization and interpretation of        molecular diagnostic tests will be granted to the resident by the laboratory director, once        he/she has demonstrated sufficient proficiency in the field. The other privileges listed above        will be immediately granted. OHSU has recently been accredited for a new "molecular genetic pathology" fellowship program (1 year in duration) in which an MGP fellow will spend at least 3 months in the DNA Diagnostic lab, in addition to rotations through Cytogenetics, Biochemical Genetics, and the pursuit of independent research.