Oregon Stem Cell Center Flow Cytometry Core
Welcome to the OSCC Flow Cytometry Core website. We hope this site will give you a better idea of who we are, what we do, and how you can better prepare for sorting or analysis.What is flow cytometry and cell sorting?
In flow cytometry, cells are passed single file through laser interrogation points. The cells, tagged with
fluorochromes on appropriate markers, scatter the light and fluoresce, which can then be read in terms of cell
morphology, surface proteins, DNA content, or cytoplasmic processes. Though this might be done with other technology,
the advantage that flow cytometry has is speed, the speed with which events can be correlated in a large population
of cells.
Cell sorting takes cytometric analysis a step further. By piezoelectrically perturbing the stream, the stream containing the cells is broken into droplets. With precise timing on a charge given the stream, it is possible to deflect a selected droplet as it passes through an electric field. When an event of interest is read, the machine is able to deflect and collect the droplet containing the event.
- Policies and hourly rates
- Getting ready to do a sort? Here are some tips.
- Two examples of how to identify stem cells
- http://www.bdbiosciences.com/spectra
- http://probes.invitrogen.com/products/flowcytometry/practicalflowcytometry.html
The OSCC Flow Cytometry Core is located in the Biomedical Research Building (BRB) Room 753H on the Marquam Hill Campus. The hours of operation are 9 am to 4 pm, Monday through Friday. Call to inquire about extended hours. The core is open to all OHSU and non-OHSU investigators, and is mostly supported by user fees.
You can check the schedule at http://facslab.calendarhost.com/cgi-bin/calweb/calweb.cgi. The lab phone number is 503-494-0661. If you wish to schedule an appointment, contact Pamela Canaday at the above phone number or e-mail canadayp@ohsu.edu. You can contact the core director, Philip Streeter, PhD, at 503-494-1762.