Confocal Microscopy Unit
at the Oregon Hearing Research Center
Director: Peter Steyger, PhD.
For more information regarding Confocal Microscopy, or
for user training,
please see the following pages and
slide presentations.
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The Oregon Hearing Research Center operates a Multi-User BioRad 1024 ES laser scanning confocal imaging system attached to an inverted Nikon Eclipse TE300 microscope. The acquisition system (LaserSharp) operates a krypton/argon laser with 3 excitation lines and simultaneous or sequential detection of emission spectra in one, two, or three 8-bit channels (PMT detectors).
The Krypton/Argon laser has three lines, 488, 568, & 647 nm, for fluorescence excitation of dye-labeled samples. This allows the use of fluorescein, rhodamine and Cy5 based dyes or any other dyes having excitation wavelengths near these laser lines. See the Fluorescent Dye emission Chart, courtesy of Lance Ladic, UBC. In addition, we can perform reflection imaging of immunogold-labeled samples. The confocal laser scanning microscope allows optical sectioning of samples up to 200 microns thick. This allows the reconstruction of 2-D and 3-D images with low background fluorescence and outstanding resolution.
Images are digitized and can be exported to other data analysis programs as *.tif files. For information on the (free) NIH-Image analysis program visit the NIH-Image homepage. To download a free PC compatible Image version from web, visit the Scion Corporation. For a version of Image that will run on any operating system, visit Image/J website.
Sigrid Myrdal is responsible for the overall operation of the Confocal Microscopy Unit, and has extensive experience in advising principal investigators in purchasing new confocal microscopy systems, or designing new experimental paradigms for their research projects including FRET, multi-photo imaging, double and triple labeling studies, and imaging live cells over long periods of time. (Get Image of Sigrid to go with the text)
Aurelie is our core confocal microscopist,
with significant experience in microscopy, tissue culture, and histochemistry.
She can advise on optimizing your sample preparation to get the most out of
your experiments on our confocal system. You can schedule time with Aurelie to
use the confocal microscope. Please email Aurelie for the
specifics and fee schedules. In addition, Aurelie can perform image processing
on a G3 Macintosh and an Octane SGI workstation. She can also archive all
images on CD, store pertinent information on our database, and provide a color
set of thumbnail prints for your notebook. More involved Image processing can
also be performed.
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Please acknowledge our technical assistance in your publications with the following boilerplate entry: Images were acquired with a BioRad MRC 1024 ES laser scanning confocal microscope (CLSM) attached to a Nikon Eclipse TE300 inverted fluorescent microsope fitted with a krypton/argon laser generating excitation lines at 488, 568 and/or 647 nm. |
For more information regarding Confocal Microscopy, or for user training,
please see the following pages and
slide presentations.
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Oregon Health Sciences University
Oregon Hearing Research Center