OHRC Faculty Member
John V. Brigande

John Brigande ( ) joined the Oregon Hearing Research Center in 2003 and currently holds the position of Assistant Professor of Otolaryngology and is jointly appointed as Assistant Professor of Cell and Developmental Biology. He earned his Ph.D. at Boston College (Chestnut Hill, MA) in 1997 after completing his undergraduate and masters work at the same institution. John's Postdoctoral Training was in Developmental Genetics (University of Texas, Austin) and later in Developmental Biology (Purdue University, West Lafayette, IN) with Donna Fekete. There he became interested in gene delivery into the developing mouse otocyst.

Research Interests

The Brigande lab vision is to establish gain and loss of function experimental paradigms to define gene function in the developing mammalian inner ear. To address this question, plasmids and viruses are injected with a micro-pipette into the developing mouse otocyst in utero. This technique enables us to evaluate embryos pre or postnatally and identify genes critical in normal and pathologic growth of the auditory mechanism.

John in the news at ohsu.

Selected Publications

For a current list of peer-reviewed publications, please visit PubMed

Text Book Chapters

Brigande, J.V., Gubbels, S.P., Woessner, D.W., Jungwirth, J.J., and Bresee, C.S. (in press). Electroporation-Mediated Gene Transfer to the Developing Mouse Inner Ear. In: Methods in Molecular Biology: Methods In Auditory and Vestibular Research, Sokolowski, B., (Ed), New Jersey: Humana Press.

Brigande, J.V. Regenerative Medicine for Inner Ear Disease. Medical Otology and Neurotology. 2006. Mohamed Hamid, M.D. & Aristides Sismanis, M.D. (Eds), New York: Thieme Medical Publishing.

Abstracts

Brigande, J.V. and Fekete, D. M. Fate map of the chick otic cup. (2000) Abstracts for the Twenty-Third Annual MidWinter Research Meeting of the Association for Research in Otolaryngology 23: 52.

Brigande, J.V., Miller, K.A., and Fekete, D.M. (2001) Misexpression of fibroblast growth factors 8 and 10 perturbs chick inner ear development. Abstracts for the Twenty-Fourth Annual MidWinter Research Meeting of the Association for Research in Otolaryngology 24: 74.

Brigande, J.V., Biesemeier, D., and Fekete, D.M. (2001) Identification of the molecular mechanisms associated with fibroblast growth factor 8b and 10-mediated dysmorphogenesis of the chick inner ear. Abstracts for the 41st Annual Midwest Developmental Biology Meeting and Singer Symposium, Society for Developmental Biology.

Brigande, J.V and Fekete, D.M. (2002) Lineage analysis of the developing mouse cochlea by transuterine injection of ecotropic retrovirus. Abstracts for the Twenty-Fifth Annual MidWinter Research Meeting of the Association for Research in Otolaryngology 25: p.

Brigande, J.V, Biesemeier, D.J., Albertin, F.E., and Fekete, D.M. (2003) Lineage analysis of the developing mouse inner ear by transuterine microinjection of an ecotropic retrovirus.

Abstracts for the Twenty-Sixth Annual MidWinter Research Meeting of the Association for Research in Otolaryngology 26: 119.

Fekete, D. M. and Brigande, J.V. (2003) "Chicken" techniques in the mouse. Mini Tutorial Oral Presentation (DMF), The Mouse as an Instrument for Ear Research Conference, September 20-22, p. 18.

Brigande, J.V. (2003) Transuterine microinjection into the developing mouse inner ear. Workshop Presentation, The Mouse as an Instrument for Ear Research Conference, The Jackson Laboratory, September 20-22.

Chang, W., Brigande, J.V., Fekete, D.M., and Wu, D.K. (2003) Unraveling the roles of BMPs in inner ear development. Contributed Talk (DKW), The Mouse as an Instrument for Ear Research Conference, September 20-22, p. 21.

Brigande, J.V. and Fekete, D.M. (2003) The mouse as an experimental embryological tool for inner ear research. The Mouse as an Instrument for Ear Research Conference, The Jackson Laboratory, September 20-22, Poster Abstracts, p.4.

Brigande, J.V. and Fekete, D.M. (2004) The use of ultrasound backscatter microscopy to access the developing mouse otocyst in utero. Twenty Seventh Annual MidWinter Research Meeting of the Association for Research in Otolaryngology 27: 65.

Gubbels, S.P., Woessner, D.W., Mitchell, J., Brigande, J.V. (2007) In Vivo Electroporation of Mouse Atonal Homolog-1 Generates Supernumerary and Ectopic Hair Cells in the Developing Mouse Inner Ear. Thirtieth Annual MidWinter Research Meeting of the Association for Research in Otolaryngology, Abstract 866.