Neurogenetics:
AD Progression Mouse Model Project
Protocols
Index of Protocols
Note to those using text-mode browsers: we wish to apologize for treating you as second class netizens in advance. We use the entities µ as a micro- symbol, ° to denote degrees, and as an unbreakable space; if these do not render correctly for you, and junk is what you actually get, this is what they are supposed to represent. If this presents a difficulty for you, please let us know so that we can accomodate you. Thank you for your patience.
Our protocols for DNA:
- for the preparation of genomic DNA from tissues.
- for the preparation of CsCl DNA.
- for mouse tail DNA extraction.
- for identifying apolipoprotein-E genotypes.
- for mutation detection by SSCP.
- for a Southern blot.
- Coming Soon: DNA Sequencing Analysis.
- Coming Soon: Real Time Quantitative PCR Analysis.
Our protocols for RNA:
- for RNA isolation using Qiagen's RNeasy.
- for RNA isolation using TRIzol.
- for a colony hybridization.
- for a Northern blot.
- for probe preparation.
Our protocols for protein:
- for a protein assay.
- for protein purification.
- for immunoprecipitation.
- for protein extraction from tissues, lymphoblasts or fibroblasts.
- for a Western blot.
Our miscellaneous protocols:
This document was created for the neurogenetics laboratory run by P. H. Reddy, Ph.D. in the NSI of OHSU. Page last updated: 19 Sept 2001.