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Microarray Resources
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BBC MIAME Supporting Materials
Ischemic preconditioning in the brain reprograms the genetic response to cerebral ischemia and mimics neuroprotective strategies reported to occur in hibernation.
Mary P. Stenzel-Poore1, Susan L. Stevens1, Zhigang Xiong5, Nikola S. Lessov2, Christina A. Harrington3, Motomi Mori4, Robert Meller5, Holly L. Rosenzweig1, Eric Tobar1, Tatyana E. Shaw1, Xiangping Chu5 and Roger P. Simon5
1Department of Molecular Microbiology and Immunology, 2Oregon Stroke Center, 3Vaccine and Gene Therapy Institute, 4Biostatistics & Bioinformatics Shared Resource Cancer Institute, Oregon Health & Science University, Portland, Oregon; 5Robert S. Dow Neurobiology Laboratories, Legacy Research, Portland, Oregon.
Supporting Materials:
Miame Checklist: BBC Miame Checklist (PDF format)
Raw Data from Affymetrix MAS 4.0: Comparisons and Absolute Analysis
Data Analysis: ANOVA and t-test Results
Final List: Final Gene List Genes that met the following criteria:
- A 2.2 fold difference of expression in the ipsilateral
hemisphere as compared to the contralateral hemisphere in at least 2 of
the
3 pools
of RNA.
- A P value of less than 0.05 using a t-test comparing
differences between hemispheres.
A gene was considered differentially regulated if both conditions
were met.
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