The cost of running a RNA sample through the AMC is broken down into four categories:
labeling charge, cost of the GeneChip array, hybridization & analysis fee, and data management fee.
Due to the experimental nature of microarray projects, samples may fail or need to be reprocessed.
When this happens, the AMC will inform you if additional charges are accrued.
For questionable samples or labeled targets at QC borderline a Test Hybridization Assay is available for $150,
which includes price of Test array and all processing and analysis.
The AMC does accept projects from non-OHSU scientists
(see below).
| Pricing Structure (per sample) |
| RNA Assessment Service |
The concentration of samples submitted to the AMC will be measured on a dedicated
spectrophotometer that is performance checked using DNA standards prior to
collecting sample measurements. Using concentration data provided by the
investigators laboratory, we will determine the most accurate method to quantitate
the RNA. Currently we routinely use both standard absorbance and fluorometric
techniques.
In addition, an aliquot of RNA is processed on the Agilent Bioanalyzer
. Using records collected since 2001 and the sample details provided on
the AMC sample submission form, we will interpret the traces and determine
if the sample is of acceptable quality for microarray analysis. These results
will be provided to all investigators associated with the project.
Cost
- $5 per sample
Note: A processing fee of $25 per group of submitted samples will be applied if the samples do not go on to be labeled as part of a GMSR project.
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| RNA Labeling Service |
This service includes the synthesis of labeled and quality checked target.
One cycle cDNA synthesis w/Affymetrix IVT (Standard labeling)
1.0 to 10 ug total RNA1 - $210 per sample*
Two cycle cDNA synthesis w/Affymetrix IVT (Small sample labeling_Affymetrix)
10ng to 100ng total RNA - $290 per sample*
NuGEN Ovation v.2 w/ FLv2 (Nano Ovation labeling_NuGEN)
5ng to 50ng total RNA - $290 per sample*
*Labeling fee includes UV measurement and bioanalysis of the nucleic
acid target for determining yield, quality & purity. Storage of
all remaining sample and prepared target for up to one year is also
included.
1The AMC can prepare labeled target form polyA+ purified RNA. Please contact us for additional information.
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| Affymetrix GeneChip Probe Array Prices (Accurate as of 03/03/2008) |
Each GeneChip genome array represents about 10,000 to 40,000 different
genes or EST clusters. For more information about Affymetrix GeneChip probe
arrays visit the Affymetrix website. The array prices below represent discounted
prices that are available to OHSU scientists through the Affymetrix Academic
Core Program which the AMC subscribes to on an annual basis. All prices
include shipping, handling and storage of chips in the AMC lab.
- Rhesus Macaque Genome Array - $460
- Human Genome HG-U133 Plus 2.0 - $460
- Human Genome HG-U133A 2.0 - $310
- Murine Genome MOE 430 2.0 - $460
- Murine Genome MOE 430A 2.0 - $310
- Murine Genome MOE 430A - $410
- Murine Genome MOE 430B - $410
- Rat Genome RAE 230 2.0 - $435
- Rat Genome RAE 230A - $385
- Rat Genome RAE 230B - $385
- C. elegans Genome Array $310
- Canine Genome Array - $310
- Chicken Genome Array - $ 435
- Drosophila Genome 2.0 - $310
- Drosophila Genome - $310
- Porcine Genome Array - $310
- Xenopus - $310
- Yeast Genome YG-S98 - $310
- Yeast Genome 2.0 - $210
- Zebrafish - $310
- New arrays released by Affymetrix but not yet listed above are generally available as well.
Contact Chris Harrington for details.
*Older Chip types are available by special order. These arrays are outlined
in the list below:
Murine MG_U74A
Rat RG_U34A,
Human HG_U95A
Human Genome HG-U133A
Human Genome HG-U133B
Human Genome Focus
Please contact Chris Harrington for details.
Note: Array prices may change with 2 weeks notice.
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| Hybridization and Data Preparation Service |
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For new samples or previously-labeled cRNA targets, array processing and
data analysis with Microarray Suite (MAS) requires the following charges:
Cost* - $135 per array
Note: For questionable samples or labeled targets at QC borderline,
a Test Hybridization Assay is available for $150. This charge includes
price of
Test array and all processing and analysis.
*Includes access to the GMSR Bioinformatics Suites at the Center for
Health and Healing (CHH) and analysis software tutorials.
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| Data Management Service |
All processing information and array performance data produced in the
AMC is loaded into the AMC Laboratory Information Management System (LIMS).
GeneChip Cel files are permanently archived in a custom Oracle database maintained
by the AMC. Additionally, the AMC makes electronic backups of all data (raw
and processed) for each project and distributes copies on DVD to the PI.
The fee for this service covers materials, software development, user support
and other operating costs. The fee applies to each array assay performed
in the AMC.
Cost - $50 per array
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| Non-OHSU Customers |
AMC services are available to scientists not affiliated with OHSU. Projects
submitted from non OHSU investigatrs will incur additional charges.
Please contact Chris
Harrington for details.
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Use the form below to get a quick
estimate of how much it will cost
run your experiment
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