Anne Raub Greenlee, PhD
Oregon Health and Science University School of Nursing
Center for Research on Occupational and Environmental Toxicology
One University Blvd
La Grande, OR 97850
greenlee@ohsu.edu
(541) 962-3389
Education
| 1989 | PhD | Washington State University, Pullman, WA |
| 1982 | MS | Washington State University, Pullman, WA |
| 1973 | BS | University of Illinois, Chicago, IL |
Employment History
| 8/04 - date | Associate Professor, Oregon Health & Science University School of Nursing, La Grande, OR and Assistant Scientist, Center for Research on Occupational and Environmental Toxicology, Portland, OR. Investigating pesticide exposures for their possible effects on early embryonic development and somatic differentiation with the long-term goal of developing interventions to safeguard populations at risk. Mentoring students and post-doctoral fellows; faculty recruitment. Teaching Occupational and Environmnental Health (CPH 539). |
| 10/01 - 6/04 | Director, Midwest Agricultural Center, Marshfield, WI. Set research agenda safeguarding the health of agricultural workers and their families. Monitored activities of the administrative, education/outreach and research cores. Interacted frequently with center staff and scientists. Represented center at national and regional meetings. Corresponded with NIOSH concerning budgets, interim and annual reports. |
| 12/00 - 6/04 | Research Scientist, Marshfield Medical Research Foundation, Marshfield, WI. Investigated agricultural exposures for their effects on reproductive and neurological health. Developed methods for identifying mechanisms of toxicant-induced injury. Participated on state committee to establish pesticide sales and use reporting system. Authored grants and journal articles; presented results at national and international meetings. Responded to media and lay requests for information on health effects of child and adult agrochemical exposures. |
| 10/96 - 12/00 | Associate Scientist, Marshfield Medical Research Foundation, Marshfield, WI. Conducted laboratory- and epidemiology-based research investigating the possible effects of occupational, residential exposures, and lifestyle choices on reproductive health (fertility, early development, pregnancy, prostate cancer). |
| 1992 - 9/96 | Postdoctoral Fellow/Clinical, Reproductive Physiology, Marshfield Medical Research Foundation, Marshfield, WI. Areas of research: preimplantation disease diagnosis, optimization of embryo culture; embryo cryopreservation. Clinical responsibilities: human IVF, andrology, endocrinology and fetal maturity. |
| 1990 - 1992 | Postdoctoral Research Associate, Department of Biological Sciences, University of Idaho, Moscow, ID. |
| Fall 1991 | Visiting Assistant Professor, Department of Biological Sciences, University of Idaho, Moscow, ID. |
| 1988 - 1990 | Postdoctoral Research Associate, Department of Animal Sciences, Washington State University, Pullman, WA. |
| 1984 - 1988 | Graduate Research Assistant, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA. |
Honors
| 2006 | Invited Lecture “Mouse Embryonic Stem Cells for Embryo Toxicity Testing” US EPA, Research Triangle Park, NC. |
| 2005 | Keynote Lecture “Human Reproduction - Environmental Health Perspectives” 8th International Symposium of G & O Barra; Rio de Janeiro, Brasil |
| 2004 | Best Scientific Poster. “Pesticides, Genetics and Risk of Parkinson Disease”. Presented at: “Cultivating a Sustainable Agricultural Workplace” Troutdale, OR |
| 2000 | American Society for Cell Biology and World Academy of Arts & Science's Emily Mudd Fellowships to attend Frontiers in Reproduction: Molecular and Cellular Concepts and Applications; Marine Biology Laboratory, Woods Hole, MA. |
Grant Support
| 2006 - 2009 | “Genetic factors involved in carcinogenesis following exposure to the pollutant benzo(a)pyrene” National Science Foundation of China $140,000 (US Project Scientist) |
| 2006 - 2008 | “Nonlethal screening method for detection of bacterial kidney disease in Pacific salmon”. Oregon Sea Grant $95,000 (Co-Investigator) |
| 2005 - 2006 | “Pesticides, Oxidative Stress and Developmental Injury” Betty Gray Rural Health Development Fund. $18,500 (Principal Investigator). |
| 2004 - 2005 | “Mitochondrial haplotypes and atovastatin-induced myopathy” Marshfield Clinic Research Foundation. $17,427 (Co-Investigator) |
| 2004 - 2007 | “Personal Monitor for Organophosphate Pesticide Exposure” $2,139,969 National Institute for Environmental Health Sciences Phase 2 SBIR (Consultant) |
| 2001 - 2004 | Midwest Center for Agriculture Research, Education & Disease Injury Prevention, $255,253. National Institute for Occupational Safety and Health (Principal Investigator). |
| 2001 - 2004 | “Pesticides, Genetics and Risk of Parkinson's Disease,” $242,463. National Institute for Occupational Safety and Health (Principal Investigator). |
| 2000 - 2003 | “Screening of Agricultural and Lawn Care Pesticides for Developmental Toxicity Using the Mouse Embryo Assay,” $36,381. Department of Agriculture, Trade and Consumer Protection (Principal Investigator) |
| 1999 - 2003 | “Molecular Epidemiology of Prostate Carcinogenesis,” $721,439 (direct costs year 01). National Cancer Institute (Co-Investigator) |
| 1997 - 2002 | “National Center for Childhood Agricultural Injury Prevention,” $608,147. Department of Health and Human Services, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, (environmental health advisor). |
| 1997 - 2001 | “Pregnancy Outcomes for Murine Embryos Exposed in vitro to Estrogenic Pesticides,” $48,862. Marshfield Medical Research Foundation (Principal Investigator). |
| 1996 - 2003 | “Fertility Outcomes for Women Working and Living in Agricultural Settings,” a program of the Midwest Center for Agricultural Disease and Injury Research, Education, and Prevention grant, ($440,000). National Institute for Occupational Safety and Health (Principal Investigator). |
| 1999 - 2000 | “Rapid Screening of Developmental Toxicant Using the Mouse Preimplantation Embryo Assay,” $14,075. Marshfield Medical Research Foundation (Principal Investigator) |
| 1998 - 2000 | “Altered Growth Factor Signaling in Murine Embryos Exposed in vitro to Estrogenic Pesticides,” $48,890. MMRF, Disease Specific Restricted Funds (Principal Investigator) |
| 1994 - 1995 | “Determination of embryo gender as a means of avoiding X-linked heritable diseases,” $12,000. Marshfield Medical Research Foundation (Co-Investigator). |
| 1991 - 1993 | “Trout myogenesis: regulation of postnatal hyperplastic and hypertrophic events,” $170,000. USDA (Co-author; Post Doctoral Fellowship support). |
Journal Review
| 2005 - present | Jornal Brasileiro de Reproducao Assistada, International Associate Editor |
| 2004 - present | Apoptosis - reviewer |
| 2004 - present | Regulatory Toxicology and Pharmacology - reviewer |
| 2003 - present | Journal of Agromedicine, Editorial Board |
| 2002 - present | Reproductive Toxicology - reviewer |
| 1999 - present | Clinical Medicine and Research, Associate Editor |
Professional Affiliations
| 2004 - present | Pacific Northwest Association of Toxicologists |
| 2003 - present | International Society for Stem Cell Research |
| 2002 - present | International Society for Environmental Epidemiology |
| 2002 - present | Teratology Society |
| 2001 - present | Society for the Study of Reproduction |
| 1999 - present | The Society of Toxicology |
| 1998 - 2004 | Midwest Regional Chapter of the Society of Toxicology |
| 1995 - present | American Association of Bioanalysts |
| 1992 - present | American Society for Reproductive Medicine |
| 1989 - present | American Association for the Advancement of Science |
Board Certification
| 1995 - | Andrology (#7109, American Board of Bioanalysts) |
| 1995 - | Embryology (#7109, American Board of Bioanalysts) |
| 1973 - | Medical Technologist (#088501, American Society of Clinical Pathologists) |
Post-Graduate Studies
| 4/06 | How to Culture Human Embryonic Stem Cells, Madison, WI |
| 3/06 | Use of Genomic Databases for Toxicology, San Diego, CA |
| 3/06 | Predictive Power of Novel Technologies (Cells to Omics), San Diego, CA |
| 3/06 | Functional Analysis of Gene and Protein Expression, San Diego, CA |
| 3/05 | Nanotechnology, Nanobiology and Nanomedicine; Portland, OR. |
| 3/05 | Fundamentals of Nanotechnology: Chemistry, Exposure, Health/Environmental Assessments, New Orleans, LA. |
| 3/05 | Developmental Toxicology Studies: Design, Interpretation and Risk Assessment; New Orleans, LA |
| 3/05 | Male Reproductive Hazard Indentification and Risk Assessment; New Orleans, LA |
| 11/04 | Writing Winning Grants; OHSU, Portland, OR. |
| 10/03 | Clinical Proteomics: Translational Applications Using Laser Capture Microdissection, NIH, Bethesda, MD |
| 8/02 | Environmental Agents and Human Reproduction: Overcoming Methodologic Challenges, Vancouver, BC |
| 6/02 | Experimental Design and Biostatistics, Scottsdale, AZ |
| 6/02 | Mechanisms, Biomarkers, Data Interpretation: An Integrative Risk Assessment using Developmental Nerotoxicity as a Model, Scottsdale, AZ |
| 12/01 | Symposium on Gene Expression and Proteomics in Environmental Health Research, Natcher Center Auditorium, National Institutes of Health, Bethesda, MD |
| 10/01 | The Role of Environmental Toxicants in Premature Birth, National Academy of Sciences, Washington, DC |
| 5/00 | Frontiers in Reproduction: Molecular and Cellular Concepts; Woods Hole, MA |
Committees
| 2005 - date | Research Council, OHSU School of Nursing |
| 2004 | Chair, CDC-NIOSH, Educational Research Centers Site Visit and SEP |
| 2004 | Reviewer. CDC-NIOSH ; Disease, Disability and Injury Prevention and Control, SEP |
| 2002 - date | Society for the Study of Reproduction, Minority Affairs Committee |
| 2000 - date | American Society for Reproductive Medicine, Women's Council |
| 1999 - 2004 | Marshfield Clinic Institutional Animal Care and Use Committee |
| 1999 - 2002 | Marshfield Clinic Research Committee |
| 1994 - 2000 | Marshfield Clinic Institutional Review Board; Co-Chair and Chair |
Teaching Responsibilities
| 2005 - date | Doctoral Committee, OHSU School of Nursing (Laura Larsson) |
| 2005 | CROET Summer Undergraduate Mentor |
| 2004 - date | OHSU School of Nursing; BSN Honors Research |
| 1992-2003 | Summer Intern Program, Marshfield Clinic Research Foundation |
| 1999-2001 | Doctoral Committee; University of Wisconsin-Madison (Fernanda Cavieres) |
| 1997-2004 | Wisconsin Science Teachers Annual Forum |
Peer-Reviewed Publications
Greenlee AR, Ellis TM, Berg RL, Mercieca MD. 2005 Pregnancy outcomes for mouse preimplantation embryos exposed in vitro to the estrogenic pesticide o,p-DDT. Reproductive Toxicology. Doi:10.1016/j.reprotox.2005.02.004
Greenlee AR, Kronenwetter-Koepel TA, Kaiser SJ, Liu K. 2005 Comparison of Matrigel and gelatin substrata for feeder-free culture of undifferentiated mouse embryonic stem cells for toxicity testing. Toxicology In Vitro 19:389-397. DOI 10.1016/j.tiv.2004.11.002
Jiang Y, Ellis TM, Greenlee AR. 2004 Genotyping Parkinson disease-associated mitochondrial polymorphisms. Clinical Medicine and Research 2:99-106.
Greenlee AR, Kronenwetter-Koepel T, Kaiser S, Ellis TM, Liu K. 2004 Combined effects of Matrigel and growth factors on undifferentiated murine embryonic stem cells for embryo toxicity testing, Toxicology In Vitro 18:543-553. DOI 10.1016/j.tiv.2004.01.013.
Greenlee AR, Ellis TM, Berg RL. 2004 Low-dose agrochemicals and lawn care pesticides induce developmental injury in murine preimplantation embryos. Environmental Health Perspectives 112:703-709. DOI number 10.1289/ehp.6774; 22 January 2004.
Giampietro PF, Blank RD, Raggio CL, Merchant S, Jacobsen FS, Faciszewski T, Shukla SK, Greenlee AR, Reynolds C, Schowalter DB: 2003 Congenital and Idiopathic Scoliosis: Clinical and Genetic Aspects. Clin Med & Res;1:125-136.
Greenlee AR, Arbuckle TE, Chyou PH. 2002 Risk factors for female infertility in an agricultural region. Epidemiology 2003;14:429-436.
Greenlee AR, Burmester JK, Hiner BC. 2002 Pesticide exposure, host susceptibility factors and risk of Parkinson's disease. WI Med J 101:23-27.
Greenlee AR, Quail CA, Berg RL. 2000. The anti-estrogen ICI 182,780 abolishes developmental injury for murine embryos exposed in vitro to o,p'-DDT. Reprod Toxicol 14:225-234.
Stueland D, Greenlee AR. 1999. Factors affecting initial cholinesterase levels from a pilot study of central Wisconsin farmers. J Ag Safety Health 5:367-371.
Greenlee AR, Quail CA, Berg RL. 1999. Developmental alterations in murine embryos exposed in vitro to an estrogenic pesticide, o,p'-DDT. Reprod Toxicol 13:555-565.
Greenlee AR, Stueland DT, Jacks JJ. 1998. Endocrine disrupting chemicals and human health implications. Marshfield Clinic Bulletin 38:3-12.
Greenlee AR, Krisher RL, Plotka ED. 1998. Rapid sexing of murine preimplantation embryos using a nested, multiplex polymerase chain reaction (PCR). Mol Reprod Dev 49:261-267.
Greenlee AR, Dodson MV, Yablonka-Reuveni Z, Kersten CA, Cloud JG. 1995. In vitro differentiation of myoblasts from skeletal muscle of rainbow trout (Oncorhynchus mykiss). J Fish Biol 46:731-747.
Greenlee AR, Kersten CA, Cloud JG. 1995. Effects of triploidy on rainbow trout myogenesis in vitro. J Fish Biol 46:381-388.
Greenlee AR, Ristow SS. 1993. A monoclonal antibody which binds a carbohydrate moiety broadly expressed on rainbow trout leukocytes. Fish and Shellfish Immunology 3:317-330.
Greenlee AR, Brown RA, Ristow SS. 1991. Nonspecific cytotoxic cells of rainbow trout kill YAC-1 targets by both necrotic and apoptic mechanisms. Devel Comp Immunol 15:153-164.
Congleton JL, Greenlee AR, Ristow SS. 1990. Isolation of leukocytes from the anterior kidney and spleen of rainbow trout in a self-generating gradient. J Fish Biol 36:575-585.
Greenlee AR, Magnuson NS, Smith C, Butt B, McKiernan AJ, Davis WC. 1990. Characterization of heteromyeloma fusion partners which promote the outgrowth of porcine hybridomas. Vet Immunol Immunopath 26:267-283.
Greenlee AR, Banks KL. 1985. Cellular and lipopolysaccharide subunit requirements for the carprine lymphoblastic response to endotoxin. Am J Vet Res 46:75-79.
Abstracts (last 4 years)
Church TR, Reding DJ, Alexander BH, Greenlee AR, Burmester JK, Greenlee RT. “Farming, triazine herbicide exposure and prostate cancer” 97th Am Assn Cancer Res meeting, Washington DC, Nov 14, 2005
Greenlee AR, Kronenwetter-Koepel TA, Kaiser SJ, Liu K. Comparison of Matrigel and gelatin substrata for feeder-free culture of undifferentiated mouse embryonic stem cells for toxicity testing. 44th Annual Meeting Society of Toxicology, March 7-10, 2005, New Orleans.
Church TR, Reding D, Alexander B, Greenlee AR, Burmester JK, Greenlee RT. Quantifying the bias due to variability in earliness of diagnosis for studies of behavioral risk factors in prostate cancer. Frontiers in Cancer Prevention Research. October 16-20, 2004, Seattle, WA.
Greenlee AR, Burmester JK, Hiner BC et al. Pesticides, Genetics and Risk of Parkinson Disease - Pilot Study Update. Cultivating a Sustainable Agricultural Workplace. September 12-14, 2004, Troutdale, OR.
Greenlee AR, Arbuckle TE, Chyou P-H: Risk factors for female infertility in an agricultural region. Fifth International Symposium, Future of Rural Peoples: Rural Economy, Healthy People, Environment, Rural Communities. October 21, 2003, Saskatoon, Saskatchewan, Canada.
Carl WR, Chyou P-H, Herr J, Kempf DL, and Greenlee AR: Potential sources of bias in a study of infertility and environmental exposures. Annual Meeting of the American College of Epidemiology, Sept 7-9, 2003, Chicago, IL.
Ellis TM, Berg RL, Mercieca MD, Greenlee AR: Pregnancy outcomes for murine embryos exposed in vitro to an estrogenic pesticide. Midwest Regional Chapter of the Society of Toxicology, May 16, 2003, Lincolnshire, IL.
Koepel T, Ellis T, Kaiser S, Greenlee AR: Characterization of Murine Embryonic Stem Cells (mESCs) cultured on MatrigelT in a feeder-free and conditioned medium-free environment. Keystone Conference, March 29-April 3, 2003, Steamboat Springs, CO.
Kaiser S, Kronenwetter-Koepel T, Greenlee AR: Biomarkers of Oxidative Stress in Murine Embryonic Stem Cells. Great Lakes International Imaging and Flow Cytometry Association, October 4-6, 2002, Detroit, MI.
Greenlee AR, Arbuckle TE, Chyou PH. 2002 Risk factors for female infertility in an agricultural region. 14th Conference of the International Society for Environmental Epidemiology, Vancouver, BC. Epidemiology 13:S247.
Greenlee AR, Ellis TM, Berg RL. 2002 Developmental injury in murine preimplantation embryos exposed in vitro to commonly used agrochemicals. 42nd Annual Meeting Teratology Society, Scottsdale, AZ. Teratology 65:310.
Greenlee AR, Ellis TL, Berg RL. 2002 Screening of Agricultural and Lawn Care Pesticides for Developmental Toxicity using the Mouse Embryo Assay. International Conference on Pesticide Exposure and Health meeting, July 8-12, 2002, NIH, Bethesda, MD.
Greenlee AR, Quail CA, Berg RL, Linneman J, Mercieca MD. 2000. Pregnancy outcomes for murine embryos exposed in vitro to the estrogenic pesticides, o,p'-DDT. Fertil Steril 74:S133-S134.
Greenlee AR, Quail CA, Misner LM, Liu K: 2000 Insulin-like growth factor expression in murine embryos exposed in vitro to o,p'-DDT. Presented at Frontiers in Reproduction Symposium, Boston, MA, June 15-17, 2000.
Greenlee AR, Eaker ED, Chyou P-H, Schwabe S, Wittman L, Bergs R: 2000 Fertility outcomes for women working and living in agricultural settings - An update. Presented at the Agricultural Safety & Health in a New Century Meeting, April 28-30, 2000, Cooperstown, NY.
Reports
Church TR, Reding DJ, Alexander BH, Greenlee AR, Burmester JK, Greenlee RT. 2005 Molecular Epidemiology of Prostate Carcinogenesis - Final Report.
Greenlee AR, Purschwitz MA. 2004 NIOSH Agricultural Centers. Final Progress Report for the Midwest Center for Agricultural Research, Education, and Disease and Injury Prevention U50 OHO7543-02.
