The Flow Cytometry Shared Resource is pleased to announce the expansion of the capacity and capability of the core with the addition of new instruments and upgrades. The resource has added a state-of the-art cell sorter, the Becton Dickinson (BD) InFlux, which is capable of detecting up to eleven fluorescent probes for cell separation and cell analyses. The instrument is equipped with four lasers, including a 561nm green laser for the highly demanded red fluorescent protein detection. This new instrument also has the same 405nm, 488nm, and 640nm lasers as our LSRII analytical instrument. With this similar configuration, investigators using the LSRII for analysis will find an easy transition to sorting when needed. This new BD Influx is temporarily located on the 0 floor of Richard Jones Hall until the completion of the new flow cytometry facility, scheduled for this spring.
The existing BD InFlux, located in the Biomedical Research Building on the 7th floor, has been upgraded with a fourth laser. It is now able to detect nine fluorescent probes and is configured with 355nm,488nm, 561nm, and 640nm lasers. Both of these instruments allow for the sterile isolation of pure cell populations from complex cell mixtures both hematopoietic and non-hematopoietic, and can be used to sort cells in bulk or for cell cloning.
The resource has also added two Miltenyi instruments, the MACSQuant and AutoMACS Pro. The MACSQuant is a 7-color analytical flow cytometer capable of analyzing cells from tubes as well as 96-well plates. The AutoMACS Pro is a cell enrichment device which will save investigator time and funding by facilitating the pre-enrichment of cells prior to InFlux sorting.
For more information, visit www.ohsu.edu/flowcytometry.