METAL ION CORE

Atomic Absorption

Mass Spectroscopy

Confocal Microscopy

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The Metal Ion Core is part of the NIH Program Project Grant GM067166. We perform analytical services for members of the core.

Types of analysis performed in this core:

Atomic Absorption Spectroscopy

The concentration of a metal in a sample (i.e. Fe or Cu) is determined by measuring the absorbance of an element specific emitted wavelength when the the sample is heated to temperatures above 1200ºC.

Confocal Microscopy

Using confocal microscopy we are able to look onto and into tissue that was stained with fluorescent stain. Co-localization studies and 3-d reconstruction of cell or cell compartments allow for pretty pictures.

ESI Mass Spectroscopy

ESI Mass Spectroscopy determines its mass of a protein in the range from 4500 - 45000 D. MS-MS scans and subsequent database search of protein fragments as they are obtained from tryptic digestion furthermore allow for identification of peptide fragments.

EXAFS Spectroscopy

EXAFS spectroscopy is used to determine the immediate coordination around a metal in a protein. Direct ligands such as histidine or cysteine can be identified and bond distance to the ligating metal can be determined to high accuracy.