Kim Neve
Senior Research Career Scientist, Research Service, VAMC
Professor, Behavioral Neuroscience, OHSU
e-mail: nevek@ohsu.edu
Kim Neve's Personal Home Page
Major Areas
Dopamine Receptors and Transporters
Previous Positions
Research Pharmacologist, VAMC
Associate Research Career Scientist, VAMC
Assistant Professor of Psychiatry, Pharmacology
Associate Professor of Behavioral Neuroscience, Psychiatry, and Physiology & Pharmacology
Education
B.A. (1979) Dana College
Ph.D. (1984) University of California at Irvine
Research Interests
My research is broadly concerned with the function of the brain dopamine system. Our focus has been on the use of genetically modified dopamine receptors expressed in cells in culture to explore the effects of dopamine, therapeutic drugs, and abused substances on the receptors, and we are now expanding into characterizing dopamine receptor function in situ -- both in neurons in culture and in vivo -- by pharmacological and genetic manipulation of the function of signal transduction molecules. An example of this work is our utilization of in vitro mutagenesis to investigate the interactions of dopamine D2 receptors with agonists and signaling pathways. One area of emphasis is on the regulation of the sensitivity of dopamine receptors, and the regulation of the responsiveness of adenylate cyclase by dopamine receptor stimulation. The techniques that we use in these investigations include co-expression and biochemical analysis of a variety of signal transduction molecules (wildtype and mutant receptors, G proteins, effector enzymes), metabolic labeling and immunoprecipitation, and confocal fluorescence microscopy.
Selected Recent Publications
Liu Y, Teeter MM, DuRand CJ, and Neve KA (2006) Identification of a Zn 2+ binding site on the dopamine D 2 receptor. Biochem Biophys Res Comm 339 (2006) 873-879.
Lan H, DuRand CJ, Teeter MM, and Neve KA (2006) Structural determinants of pharmacological specificity between D 1 and D 2 dopamine receptors. Mol Pharmacol 69:185-194.
Wang C, Buck DC, Yang R, Macey TA, and Neve KA (2005) Dopamine D2 receptor stimulation of mitogen-activated protein kinases mediated by cell type-dependent transactivation of receptor tyrosine kinases. J Neurochem 93:899-909 .
Macey TA, Gurevich VV, Neve KA (2004) Preferential Interaction between the dopamine D2 receptor and arrestin2 in neostriatal neurons. Mol Pharmacol 66:1635-42.
Macey TA, Liu Y, Gurevich VV, and Neve KA (2005) Dopamine D1 receptor interaction with arrestin3 in neostriatal neurons. J Neurochem 93:128-134 .
Neve KA , Seamans JK, Trantham-Davidson H (2004) Dopamine receptor signaling. J Recept Signal Transduct Res. Aug;24(3):165-205.
Mason JN, Kozell LB, Neve KA (2002) Regulation of dopamine D 1 receptor trafficking by protein kinase A-dependent phosphorylation. Mol Pharmacol 61:806-16.
Watts VJ, Taussig R, Neve RL, Neve KA (2001) Dopamine D2 receptor-induced heterologous sensitization of adenylyl cyclase requires G a s: characterization of G a s-insensitive mutants of adenylyl cyclase V. Mol Pharmacol 60:168-72.
Neve KA , Cumbay MG, Thompson KR, Yang R, Buck DC, Watts VJ, DuRand CJ, Teeter MM (2001) Modeling and mutational analysis of a putative sodium-binding pocket on the dopamine D2 receptor. Mol Pharmacol 60:373-81.
Wilcox RE, Huang WH, Brusniak MY, Wilcox DM, Pearlman RS, Teeter MM, DuRand CJ, Wiens BL, Neve KA (2000) CoMFA-based prediction of agonist affinities at recombinant wild type versus serine to alanine point mutated D2 dopamine receptors. J Med Chem 43:3005-19.